The Easy Guide to Making a Dichotomous Key with Editable Examples

In the field of biology, classification plays a major role. With new species being discovered every day, it’s important to have techniques in place to identify and classify them. One such tool is the dichotomous key. It helps identify organisms by directing the user to look at the known organisms. 

In this simple guide, we will explain what is a dichotomous key and how to create one. Some examples are provided in the dichotomous key examples section; you can use any template to start your project right away. Download them as PNGs, JPEGs, SVGs or PDFs for publishing, printing, and sharing.

What is a Dichotomous Key

Students and professionals use the dichotomous key to identify and classify objects (i.e. people, animals, plants, bacteria, etc.) into specific categories based on their characteristics. It’s the most commonly used form of classification or type of identification key used in biology as it simplifies identifying unknown organisms.

Simply put, it is a method used to identify a species by answering a series of questions based on contrasting features (eg: physical characteristics) that have two possible outcomes.

“Dichotomous” means divided into two parts, hence the dichotomous keys always present two choices based on the key characteristics of the organism in each step. By correctly selecting the right choice at each stage, the user will be able to identify the name of the organism at the end. The further you divide the key, the more you learn about the specimen you are trying to identify.

When creating a dichotomous key, both qualitative (i.e. physical attributes such as how the organism looks, what color it is, etc.) and quantitative (i.e. the number of legs, weight, height, etc.) factors are considered.

It can be done in both a graphical (as a branching flowchart) or written format (series of paired statements organized sequentially).  Most often, they are used to identify plant and animal species, although it can be used to classify any object that can be identified by a set of observable characteristics.

To easily draw connections between species to better establish proposed phylogeny create Cladograms.

What is the dichotomous key used for

A dichotomous key is usually used for 

  • Identifying and categorizing organisms 
  • Helping students easily understand harder scientific concepts
  • Organizing large amounts of information to make identification of an organism much easier

How to Make a Dichotomous Key  

Below we have listed the steps you need to follow when creating a dichotomous key. 

Step 1: List down the characteristics

Pay attention to the specimens you are trying to identify with your dichotomous key. List down the characteristics that you can notice. For example, say you are trying to classify a group of animals. You may notice that some have feathers whereas others have legs, or some have long tails and others don’t. 

Step 2: Organize the characteristics in order

When creating your dichotomous key, you need to start with the most general characteristics first, before moving to the more specific ones. So it helps to have identified the more obvious and less obvious contrasting characteristics among the specimen before creating your dichotomous key. 

Step 3: Divide the specimens

You can use statements (i.e. has feathers and no feathers) or questions (does it have feathers?) to divide your specimens into two groups. The first differentiation should be made on the most general characteristic. 

Step 4: Divide the specimen even further

Based on the next contrasting characteristic, divide the specimen further. For example, first, you may have grouped your animals as have feathers and have no feathers, in which case the ones with feathers can be categorized as birds while you can further subdivide the ones that have no feathers as having fur and having no fur. Continue to subdivide your specimen by asking enough questions until you have identified and named all of them.   

Step 5:  Draw a dichotomous key diagram

You can either create a text-based dichotomous key or a graphical one where you can even use images of the specimen you are trying to identify. Here you can use a tree diagram or a flowchart as in the examples below. 

Step 6: Test it out 

Once you have completed your dichotomous key, test it out to see if it works. Focus on the specimen you are trying to identify and go through the questions in your dichotomous tree to see if you get it identified at the end. If you think the questions in your dichotomous key needs to be rearranged, make the necessary adjustments. 

Best practices to keep in mind

  • Consider only one characteristic at a time 
  • Use morphological or observable characteristics as much as you can 
  • Use major characteristics when dividing the organisms in the beginning and use lesser or less obvious characteristics to divide them into smaller groups
  • When writing contrasting statements, rely on similar word formats (i.e. have feathers and don’t have feathers) 
  • Be specific in your statements and avoid repeating the same characteristics
  • Use questions that lead to yes or no answers rather than statements

To draw connections between species to better establish evolutionary connections use a Phylogenetic Tree Maker

Dichotomous Key Examples

Let’s look at some examples to make more sense of what is a dichotomous key.

Dichotomous key for animals

Dichotomous Key for Animals What is a Dichotomous Key
Dichotomous Key for Animals (Click on the template to edit it online)

Dichotomous key for insects

Dichotomous Key for Insects What is a Dichotomous Key
Dichotomous Key for Insects (Click on the template to edit it online)

Dichotomous key for plants

Dichotomous Key for Plants What is a Dichotomous Key
Dichotomous Key for Plants (Click on the template to edit it online)

Dichotomous key for leaves

 Dichotomous Key for Leaves
Dichotomous Key for Leaves (Click on the template to edit it online)

Any More Tips on Making a Dichotomous Key?

We hope that this guide will help you familiarize yourself with the dichotomous key method. Make use of the editable templates to get a headstart in class. Invite your friends/ students to edit them online, and make a fun group activity out of it.

Any more useful tips on creating a dichotomous key that our readers can rely on? Do share them in the comments section below.

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Amanda Athuraliya

Amanda Athuraliya is the communication specialist/content writer at Creately, online diagramming and collaboration tool. She is an avid reader, a budding writer and a passionate researcher who loves to write about all kinds of topics.


  1. Nicole

    This was really helpful.

  2. chasity eloby

    i love the articule and it was very hepful for when i went to school

  3. whitney zindoga

    thank you so much….this article is so simplified and understandable…..itwas so helpful to me…..thank you once more

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